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Laboratory of Root Physiology

Russian version

Head Ilya V. Seregin, D.Sc. (biol.)
Tel.: (499) 678-53-24, E-mail: ilya_seregin@mail.ru

Ilya V. SereginViktor B. Ivanov
Ilya V. SereginProfessor
Viktor B. Ivanov

Brief history of the Laboratory

In 1933, in order to study root nutrition in plants, A.A. Rikhter set up a group that in 1935 was reorganized into an independent Laboratory of Mineral Nutrition. In 1949, it was renamed the Laboratory of Root Nutrition. During this time span, the Laboratory was headed by such outstanding scientists as D.A. Sabinin, E.M. Ratner, Z.I. Zhurbitskii, I.I. Kolosov, D.B. Vakhmistrov, and V.B. Ivanov. The Laboratory conducted numerous investigations of the mechanisms of ion uptake by the root system, relationship between uptake and transpiration, the role of individual tissues in ion uptake and translocation, metabolism of phosphorus in plants, and root electrophysiology. Close attention was paid to hydroponics, optimization of fertilizing, and many other important applied aspects. V.B. Ivanov encouraged investigation of cellular mechanisms of root growth, effect of cytostatics, inhibitors and heavy metals on root growth , as well as development of histochemical methods. This line of research is successfully pursued today. The results of investigations were published in the monographs by I.I. Kolosov Absorbing Activity of Plant Root System (1937), E.M. Ratner Nutrition and Application of Fertilizers (1947), Z.I. Zhurbitskii Physiological and Agrochemical Bases for Application of Fertilizers (1963), in XLIX Timiryazev Lecture Spatial Organization of Ion Transport in the Root delivered by D.B. Vakhmistrov (1991), as well as in V.B. Ivanovs monographs Cellular Bases for Plant Growth (Moscow, Nauka, 1974), Reactive Dyes in Biology (Moscow, Nauka, 1982; Harwood Acad. Publ., London, 1987), Cell Proliferation in Plants (VINITI, Moscow, 1987), and Timiryazev Lecture Cellular Mechanisms of Plant Growth delivered in 2007 and published in 2011.

Since 2015, the Laboratory has been headed by Doctor of Biology I.V. Seregin who is engaged in the investigation of toxic effect of heavy metals on plants as well as their distribution and translocation. To this end, histochemical methods for cadmium, lead, nickel, zinc, and strontium detection in plant tissues were developed or modified in the Laboratory. Special attention is paid to investigation of physiological mechanisms of selective accumulation of heavy metals in two contrasting groups of plants: excluders that predominantly accumulate metals in the roots and hyperaccumulators that accumulate metals mainly in the shoots.

In 2000, the Laboratory was reinforced with a group headed by M.S. Krasavina who is engaged in the investigation of phloem transport and electrophysiology.

Major lines of research

Presently, the Laboratory conducts investigationsin the following fields:

  1. Cellular mechanisms of root growth;
  2. Patterns of root growth under the effect of various agents;
  3. Root stem cells;
  4. Effect of heavy metals on plant growth and development of the methods for their histochemical detection in plant tissues;
  5. Distribution and accumulation of heavy metals in excluders and hyperaccumulators;
  6. Possible mechanisms of selective accumulation of metals in underground organs of excluders and above-ground organs of hyperaccumulators;
  7. Mechanism of phloem unloading and loading and metabolism of carbohydrates in the root.

Methodological resources

We elaborated the methods for biological testing based on the effect of certain compounds on root growth. Histochemical methods for detection of cadmium, lead, nickel, and zinc in plant tissues, for determination of invertase and sucrose synthase activity, as well asfor staining proteins and carbohydrates with procion dyes were developed in the Laboratory. We employ computerized methods of bright-field and fluorescent microscopy. We worked out methods for visualization of phloem unloading using fluorescent dyes.


The Laboratory cooperates with the Department of Genetics, Vrije Universiteit Amsterdam; Laboratory of Genetics and Evolution of Plant Populations, University of Lille 1; Department of Botany, University of Klaipeda; Laboratory of Biochemistry and Molecular Plant Physiology, Montpellier SupAgro; Department of Biology, University of Eastern Finland, Kuopio Campus.

Research Staff

Ilya V. Seregin D.Sc. (biol.), Head of the Laboratory
Tel.: (499) 678-53-24, E-mail: ilya_seregin@mail.ru

Victor B. Ivanov, D.Sc. (biol.), Professor, Leading Researcher
Tel.: (499) 678-53-04, E-mail: ivanov@ippras.ru

Ilya A. Baklanov Ph.D. (biol.), Research Associate
Tel.: (499) 678-53-24, E-mail: baklanov_ia@list.ru

Nataliya A. Burmistrova, Research Associate
Tel.: (499) 678-53-71, E-mail: na_burmistrova@ippras.ru

Elena I. Bystrova, Ph.D. (biol.), Senior Researcher
Tel.: (499) 678-54-00, E-mail: ivanov@ippras.ru

Natalia V. Zhukovskaya, Ph.D. (biol.), Research Associate
Tel.: (499) 678-53-76, E-mail: zhukovskayanv@rambler.ru

Anna D. Kozhevnikova , Ph.D. (biol.), Senior Researcher
Tel.: (499) 678-53-24, E-mail: urtica8127@yandex.ru

Marina S. Krasavina , Ph.D. (biol.), Senior Researcher
Tel.: (499) 678-53-71, E-mail: krasavina@ippras.ru

Nina F. Lunkova (Feshchenko), Ph.D. (biol.), Research Associate
Tel.: (499) 678-53-71, E-mail: krasavina@ippras.ru

Nadezhda P. Nyukhacheva, Engineer
Tel.: (499) 678-53-71, E-mail: ivanov@ippras.ru

Selected publications 2001-2016

Pacheco-Escobedo M.A., Ivanov V.B., Ransom-Rodriguez I., Arriaga-Mejia G., Avila H., Baklanov I.A., Pimentel A., Corkidi G., Doerner P., Dubrovsky J.G., Alvarez-Buylla E.R., Garay-Arroyo A. (2016) Longitudinal zonation pattern in Arabidopsis root tip defined by a multiple structural change algorithm. Annals of Botany, 118(4), 763-776.

Seregin I.V., Kozhevnikova A.D., Schat H. (2015) Histochemical Detection of Zn in Plant Tissues. Bio-protocol., 5(10), pii: e1470

Kozhevnikova A.D., Erlikh N.T., Zhukovskaya N.V., Obroucheva N.V., Ivanov V.B., Belinskaya A.A., Khutoryanskaya M.Y., Seregin I.V. (2014) Nickel and zinc effects, accumulation and distribution in ruderal plants Lepidium ruderale and Capsella bursa-pastoris. Acta Physiologiae Plantarum, 36, 32913305.

Kozhevnikova A.D., Seregin I.V., Erlikh N.T., Shevyreva T.A., Andreev I.M., Verweij R., Schat H. (2014) Histidine-mediated xylem loading of zinc is a species-wide character in Noccaea caerulescens. New Phytologist, 203, 508-519.

Kozhevnikova A.D., Seregin I.V., Verweij R., Schat H. (2014) Histidine promotes the loading of nickel and zinc, but not of cadmium, into the xylem in Noccaea caerulescens. Plant Signaling and Behavior, 9, pii: e29580.

Chaney R. L., Reeves R.D., Baklanov I.A., Centofanti T., Broadhurst C.L., Baker A.J.M., van der Ent A., Roseberg R.J. (2014) Phytoremediation and Phytomining: using plants to remediate contaminated or mineralized environments. In: Plant Ecology and Evolution in Harsh Environments. Nishanta Rajakaruna, Robert S. Boyd, Tanner B. Harris, eds. Nova Science Publishers, 365392.

Onipchenko V.G., Kipkeev A.M., Makarov M.I., Kozhevnikova A.D., Ivanov V.B., Soudzilovskaia N.A., Tekeev D.K., Salpagarova F.S., Werger M.J.A., Cornelissen J.H.C. (2014) Digging deep to open the white black box of snow root phenology. Ecological Research, 29, 529-534.

Rakhmankulova Z.F., Voronin P.YU., Shuyskaya E.V., Kuznetsova N.A., Zhukovskaya N.V., Toderich K.N. (2014) Effect of NaCl and isoosmotic polyethylene glycol stress on gas exchange in shoots of the 4 xerohalophyte Haloxylon aphyllum (Chenopodiaceae). Photosynthetica, 52, 437-443.

Krasavina M.S., Burmistrova N.A., Raldugina G.N. (2014) The Role of Carbohydrates in Plant Resistance to Abiotic Stresses. P. Ahmad., S.Rassol, eds. In: Emerging Technologies and Management of Crop Stress Tolerance, Biological Techniques, 1, 229-270.

Krasavina M.S., Burmistrova N.A. (2013) Impact of salicylic acid on the transport and distribution of sugars in plants. In: Salicylic Acid: Plant Growth and Development. Shamsul Hayat, Aqil Ahmad, Mohammed Naseer Alyemeni, eds. Springer-Dordrecht Heidelberg New York London, 83-117.

Ivanov V.B., Dubrovsky I.G. (2013) Longitudinal zonation pattern in plant roots: conflicts and solutions. Trends in Plant Science, 18, 237242.

Krasavina M.S., Burmistrova N.A. (2011) Potassium and sugar accumulation in plant cells. In: Soil Nutrients, Miransari M, ed. Oxford: Nova Science Publishers, 21-76.

Krasavina M.S., Prudnikov G.A. (2011) Plant cold tolerance and bioelectrogenesis In: Structiral and functional deviations from normal growth and development of plants, Novitskaya L.L., ed. Petrozavodsk, 142-147.

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Richau K.H., Kozhevnikova A.D., Seregin I.V., Vooijs R., Koevoets P.L.M., Smith J.A.C., Ivanov V.B., Schat H. (2009) Chelation by histidine inhibits the vacuolar sequestration of nickel in roots of the hyperaccumulator, Thlaspi caerulescens. New Phytol., 183, 106-116.

Seregin I.V., Kozhevnikova A.D. (2009) Transport and distribution of nickel in higher plants. In: Nickel in Relation to Plants, Barket Ali, S. Hayat, A. Ahmad (eds.), Narosa Publising House Pvt. Ltd., 200 p.

Onipchenko V.G., Makarov M.I., van Logtestijn R.S.P., Ivanov V.B., Akhmetzhanova A.A., Tekeev D.K., Ermak A.A., Salpagarova F.S., Kozhevnikova A.D., Cornelissen J.H.C. (2009) New nitrogen uptake strategy: specialized snow roots. Ecology Lett., 12, 758-764.

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Krasavina M.S. (2006) Effect of salicylic acid on solute transport in plants. In: Salicylic Acid a Plant Hormone, Hayat S., Ahmad A. (eds.), Springer, pp. 122-163.

Berkovich Yu.A., Smolyanina S.O., Krivobok N.M., Erokhin A.N., Ivanov V.B. (2005) Impact of the altered light vector relative to gravity vector on plant growth and development. Adv. Spice Research, 36, 1319-1328.

Amtmann ., Armengaud ., Volkov V. (2004) Potassium nutrition and salt stress. Annu. Plant Rev., 15.

Hills A., Volkov V. (2004) Electrophysiology equipment and software. Annu. Plant Rev., 15.

Dubrovsky G. J., Ivanov V.B. (2003) Celebrating 50 years of the cell cycle. Nature, 426, 759.

De Boer A.H., Volkov V. (2003) Logistics of water and salt transport through the plant: structure and functioning of the xylem. Plant Cell Environ., 26, 87-101.

Van Ieperen W., Volkov V.S., Meeteren U.V. (2003) Distribution of xylem hydraulic resistance in fruiting truss of tomato influenced by water stress. J. Exp. Bot., 54, 317-324.

Grif V.G., Ivanov V.B., Machs E.M. (2002) Cell cycle and its parameters in flowering plants. , 44, 936-980.

Ivanov V.B., Dobrochaev A.E., Baskin T.I. (2002) What the distribution of cell lengths in the root meristem does and does not reveal about cell division. J. Plant Growth Regul., 21, 60-67.

Van der Weele C.M., Jang H.S., Palaniappan K.S., Ivanov V.B., Palaniappan K., Baskin T.I. (2002) A new algorithm for computional image analysis of deformable motion at high spatial and temporal resolution applied to root growth. Roughly uniform elongation in the meristem and also, after an abrupt acceleration, in the elongation zone. Plant Physiol., 132, 1138-1148.

Dobrochaev A.E., Ivanov V.B. (2001) Variations in the size of mitotic cells in the root meristem. Russ. J. Dev. Biol., 32, 252-262.

Obroucheva N.V., Ivanov V.B., Sobotik M., Bergmann H., Antipova O.V., Bystrova E.I., Seregin I.V., Shpigun L.K. (2001) Lead effects on cereal roots in terms of cell growth, root architecture and metal accumulation. In: Recent Advances of Plant Root Structure and Function, Gasparikova O. et al. (eds.), Kluwer Academic Publishers, pp. 165-170.